Multiplex amplification of short tandem repeat loci

DC CAFC

US 7,008,771 B1
Filed: 09/06/2002
Issued: 03/07/2006
Est. Priority Date: 09/30/1994
Status: Expired due to Fees

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First Claim

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1. A kit for simultaneously analyzing a set of loci of genomic DNA, comprising oligonucleotide primers for co-amplifying a set of loci of the genomic DNA to be analyzed, wherein the primers are in one or more containers, wherein the primers are designed to co-amplify a set of at least sixteen loci which can be co-amplified, comprisingD3S1358, HUMTH01, D21S11, D18S51, G475, Amelogenin, HUMvWFA31, D8S1179, HUMTPOX, HUMFIBRA, D5S818, D7S820, D13S317, D16S539, HUMCSF1PO, and S159.

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Abstract

Methods and materials are disclosed for use in simultaneously amplifying at least thirteen loci of genomic DNA in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of at least thirteen short tandem repeat loci, including specific materials and methods for the analysis of thirteen such loci specifically selected by the United States Federal Bureau of Investigation as core loci for use in the Combined DNA Index System (CODIS) database.

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6 Claims

1. A kit for simultaneously analyzing a set of loci of genomic DNA, comprising oligonucleotide primers for co-amplifying a set of loci of the genomic DNA to be analyzed, wherein the primers are in one or more containers, wherein the primers are designed to co-amplify a set of at least sixteen loci which can be co-amplified, comprisingD3S1358, HUMTH01, D21S11, D18S51, G475, Amelogenin, HUMvWFA31, D8S1179, HUMTPOX, HUMFIBRA, D5S818, D7S820, D13S317, D16S539, HUMCSF1PO, and S159.
- View Dependent Claims (3)
- - 3. The kit of claim 1, wherein at least one oligonucleotide primer has a sequence selected from one of the groups of primer sequences consisting of:
    - SEQ ID NO;
      
      68, SEQ ID NO;
      
      69, and SEQ ID NO;
      
      106, for the locus D3S1358;
      
      SEQ ID NO;
      
      37, SEQ ID NO;
      
      38, SEQ ID NO;
      
      66, SEQ ID NO;
      
      67, and SEQ ID NO;
      
      103, for the locus HUMTH01;
      
      SEQ ID NO;
      
      64 and SEQ ID NO;
      
      65, for the locus D21S11;
      
      SEQ ID NO;
      
      62, SEQ ID NO;
      
      63, SEQ ID NO;
      
      101, and SEQ ID NO;
      
      102, for the locus D18S51;
      
      SEQ ID NO;
      
      88, SEQ ID NO;
      
      89, and SEQ ID NO;
      
      94, for the locus G475;
      
      SEQ ID NO;
      
      86, SEQ ID NO;
      
      87, and SEQ ID NO;
      
      105, for the Amelogenin locus;
      
      SEQ ID NO;
      
      39, SEQ ID NO;
      
      40, SEQ ID NO;
      
      59, SEQ ID NO;
      
      60, and SEQ ID NO;
      
      76, for the locus HUMvWFA31;
      
      SEQ ID NO;
      
      74, SEQ ID NO;
      
      75, and SEQ ID NO;
      
      104, for the locus D8S1179;
      
      SEQ ID NO;
      
      35, SEQ ID NO;
      
      36, SEQ ID NO;
      
      72, and SEQ ID NO;
      
      73, for the locus HUMTPOX;
      
      SEQ ID NO;
      
      70, SEQ ID NO;
      
      71, and SEQ ID NO;
      
      107, for the locus HUMFIBRA;
      
      SEQ ID NO;
      
      5, SEQ ID NO;
      
      6, SEQ ID NO;
      
      84, and SEQ ID NO;
      
      85, for the locus D5S818;
      
      SEQ ID NO;
      
      1, SEQ ID NO;
      
      2, SEQ ID NO;
      
      80, and SEQ ID NO;
      
      81, for the locus D7S820;
      
      SEQ ID NO;
      
      3, SEQ ID NO;
      
      4, SEQ ID NO;
      
      82, and SEQ ID NO;
      
      83, for the locus D13S317;
      
      SEQ ID NO;
      
      29, SEQ ID NO;
      
      30, SEQ ID NO;
      
      58, SEQ ID NO;
      
      79, and SEQ ID NO;
      
      97, for the locus D16S539;
      
      SEQ ID NO;
      
      33, SEQ ID NO;
      
      34, SEQ ID NO;
      
      77, SEQ ID NO;
      
      78, and SEQ ID NO;
      
      98, for the locus HUMCSF1PO; and
      
      SEQ ID NO;
      
      90, SEQ ID NO;
      
      91, SEQ ID NO;
      
      92, SEQ ID NO;
      
      93, SEQ ID NO;
      
      95, and SEQ ID NO;
      
      96, for the locus S159.

2. A kit for simultaneously analyzing a set of loci of genomic DNA, comprising oligonucleotide primers for co-amplifying a set of loci of the genomic DNA to be analyzed, wherein the primers are in one or more containers, wherein the primers are designed to co-amplify a set of at least sixteen loci which can be co-amplified, comprising D3S1358, HUMTH01, D21S11, D18S51, S159, Amelogenin, HUMvWFA31, D8S1179, HUMTPOX, HUMFIBRA, D5S818, D7S820, D13S317, D16S539, HUMCSF1PO, and C221.
- View Dependent Claims (4)
- - 4. The kit of claim 2, wherein at least one oligonucleotide primer has a sequence selected from one of the groups of primer sequences consisting of:
    - SEQ ID NO;
      
      68, SEQ ID NO;
      
      69, and SEQ ID NO;
      
      106, for the locus D3S1358;
      
      SEQ ID NO;
      
      37, SEQ ID NO;
      
      38, SEQ ID NO;
      
      66, SEQ ID NO;
      
      67, and SEQ ID NO;
      
      103, for the locus HUMTH01;
      
      SEQ ID NO;
      
      64 and SEQ ID NO;
      
      65, for the locus D21S11;
      
      SEQ ID NO;
      
      62, SEQ ID NO;
      
      63, SEQ ID NO;
      
      101, and SEQ ID NO;
      
      102, for the locus D18S51;
      
      SEQ ID NO;
      
      88, SEQ ID NO;
      
      89, and SEQ ID NO;
      
      94, for the locus S159;
      
      SEQ ID NO;
      
      86, SEQ ID NO;
      
      87, and SEQ ID NO;
      
      105, for the Amelogenin locus;
      
      SEQ ID NO;
      
      39, SEQ ID NO;
      
      40, SEQ ID NO;
      
      59, SEQ ID NO;
      
      60, and SEQ ID NO;
      
      76, for the locus HUMvWFA31;
      
      SEQ ID NO;
      
      74, SEQ ID NO;
      
      75, and SEQ ID NO;
      
      104, for the locus D8S1179;
      
      SEQ ID NO;
      
      35, SEQ ID NO;
      
      36, SEQ ID NO;
      
      72, and SEQ ID NO;
      
      73, for the locus HUMTPOX;
      
      SEQ ID NO;
      
      70, SEQ ID NO;
      
      71, and SEQ ID NO;
      
      107, for the locus HUMFIBRA;
      
      SEQ ID NO;
      
      5, SEQ ID NO;
      
      6, SEQ ID NO;
      
      84, and SEQ ID NO;
      
      85, for the locus D5S818;
      
      SEQ ID NO;
      
      1, SEQ ID NO;
      
      2, SEQ ID NO;
      
      80, and SEQ ID NO;
      
      81, for the locus D7S820;
      
      SEQ ID NO;
      
      3, SEQ ID NO;
      
      4, SEQ ID NO;
      
      82, and SEQ ID NO;
      
      83, for the locus D13S317;
      
      SEQ ID NO;
      
      29, SEQ ID NO;
      
      30, SEQ ID NO;
      
      58, SEQ ID NO;
      
      79, and SEQ ID NO;
      
      97, for the locus D16S539;
      
      SEQ ID NO;
      
      33, SEQ ID NO;
      
      34, SEQ ID NO;
      
      77, SEQ ID NO;
      
      78, and SEQ ID NO;
      
      98, for the locus HUMCSF1PO; and
      
      SEQ ID NO;
      
      99 and SEQ ID NO;
      
      100, for the locus C221.

5. A kit for simultaneously analyzing a set of loci of genomic DNA, comprising oligonucleotide primers for co-amplifying a set of loci of the genomic DNA to be analyzed, wherein the primers are in one or more containers, wherein the primers are designed to co-amplify a set of loci from one or more DNA samples, comprising short tandem repeat loci D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, HUMCSF1PO, HUMFIBRA, HUMTH01, HUMTPOX, HUMvWFA31, and a locus selected from the group consisting of G475, S159, C221, and Amelogenin.
- View Dependent Claims (6)
- - 6. The kit of claim 5, wherein at least one oligonucleotide primer has a sequence selected from one of the groups of primer sequences consisting of:
    - SEQ ID NO;
      
      62, SEQ ID NO;
      
      63, SEQ ID NO;
      
      101, and SEQ ID NO;
      
      102, when one of the loci in the set is D18S51;
      
      SEQ ID NO;
      
      64 and SEQ ID NO;
      
      65, for the locus D21S11;
      
      SEQ ID NO;
      
      37, SEQ ID NO;
      
      38, SEQ ID NO;
      
      66, SEQ ID NO;
      
      67, and SEQ ID NO;
      
      103, for the locus HUMTH01;
      
      SEQ ID NO;
      
      68, SEQ ID NO;
      
      69, and SEQ ID NO;
      
      106, for the locus D3S1358;
      
      SEQ ID NO;
      
      70, SEQ ID NO;
      
      71, and SEQ ID NO;
      
      107, for the locus HUMFIBRA;
      
      SEQ ID NO;
      
      35, SEQ ID NO;
      
      36, SEQ ID NO;
      
      72, and SEQ ID NO;
      
      73, for the locus HUMTPOX;
      
      SEQ ID NO;
      
      74, SEQ ID NO;
      
      75, and SEQ ID NO;
      
      104, for the locus D8S1179;
      
      SEQ ID NO;
      
      39, SEQ ID NO;
      
      40, SEQ ID NO;
      
      59, SEQ ID NO;
      
      60, and SEQ ID NO;
      
      76, for the locus HUMvWFA31;
      
      SEQ ID NO;
      
      33, SEQ ID NO;
      
      34, SEQ ID NO;
      
      77, SEQ ID NO;
      
      78, and SEQ ID NO;
      
      98, for the locus HUMCSF1PO;
      
      SEQ ID NO;
      
      29, SEQ ID NO;
      
      30, SEQ ID NO;
      
      58, SEQ ID NO;
      
      79, and SEQ ID NO;
      
      97, for the locus D16S539;
      
      SEQ ID NO;
      
      1, SEQ ID NO;
      
      2, SEQ ID NO;
      
      80, and SEQ ID NO;
      
      81, for the locus D7S820;
      
      SEQ ID NO;
      
      3, SEQ ID NO;
      
      4, SEQ ID NO;
      
      82, and SEQ ID NO;
      
      83, for the locus D13S317;
      
      SEQ ID NO;
      
      5, SEQ ID NO;
      
      6, SEQ ID NO;
      
      84, and SEQ ID NO;
      
      85, for the locus D5S818;
      
      SEQ ID NO;
      
      88, SEQ ID NO;
      
      89, and SEQ ID NO;
      
      94, for the locus G475;
      
      SEQ ID NO;
      
      90, SEQ ID NO;
      
      91, SEQ ID NO;
      
      92, SEQ ID NO;
      
      93, SEQ ID NO;
      
      95, and SEQ ID NO;
      
      96, for the locus S159;
      
      SEQ ID NO;
      
      99 and SEQ ID NO;
      
      100, for the locus C221; and
      
      SEQ ID NO;
      
      86, SEQ ID NO;
      
      87, and SEQ ID NO;
      
      105, for the Amelogenin locus.

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Current Assignee
Promega Corporation
Original Assignee
Promega Corporation
Inventors
Schumm, James W., Sprecher, Cynthia J.
Primary Examiner(s)
GOLDBERG, JEANINE ANNE

Application Number

US10/236,577
Time in Patent Office

1,278 Days
Field of Search

435/6, 435/91.1, 435/91.2, 536/23.1, 536/24.3
US Class Current

435/6.12
CPC Class Codes

C12Q 1/686   Polymerase chain reaction [...

C12Q 1/6876   Nucleic acid products used ...

C12Q 2535/125   Allele specific primer exte...

C12Q 2537/143   Multiplexing, i.e. use of m...

C12Q 2563/107   fluorescence

C12Q 2600/156   Polymorphic or mutational m...

C12Q 2600/16   Primer sets for multiplex a...

Multiplex amplification of short tandem repeat loci

First Claim

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Abstract

52 Citations

6 Claims

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Multiplex amplification of short tandem repeat loci

First Claim

1 Assignment

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Litigations

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Abstract

52 Citations

6 Claims

Specification

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Solutions

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