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Process for analyzing length polymorphisms in DNA regions

DC CAFC
  • US RE37,984 E1
  • Filed: 06/09/2000
  • Issued: 02/11/2003
  • Est. Priority Date: 10/11/1988
  • Status: Expired due to Term
First Claim
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1. A method for determining length polymorphisms in a simple or cryptically simple sequence in one or more DNA regions of one or more subjects, which comprises:

  • a) providing at least one DNA sample, comprising a template DNA having a nucleotide sequence that includes a simple or cryptically simple sequence comprising trinucleotide repeats, from at least one subject;

    b) annealing at least one primer pair to the template DNA of each of said DNA samples, wherein said primer pair is composed of a first primer complementary to a nucleotide sequence flanking the simple or cryptically simple DNA sequence on the 5′

    side of said simple or cryptically simple DNA sequence and a second primer complementary to a nucleotide sequence flanking the simple or cryptically simple DNA sequence on the 3′

    side of said simple or cryptically simple DNA sequence;

    wherein said first and second primers each anneal to a single site in said template DNA and the sequence of the template DNA between the sites where said primers anneal is 50 to 500 nucleotides in length;

    c) performing at least one primer-directed polymerase chain reaction upon said template DNA having said primers annealed thereto, so as to form at least one polymerase chain reaction product;

    d) separating the products of each polymerase chain reaction according to their lengths; and

    e) analyzing the lengths of the separated products to determine the length polymorphisms of the simple or cryptically simple sequences.

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