Compositions and methods for simultaneous detection of volatile sulfur compounds and polyamines

US 20070259332A1
Filed: 12/11/2006
Published: 11/08/2007
Est. Priority Date: 10/18/2002
Status: Active Grant

First Claim

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Abstract

Disclosed are compositions and methods useful for the rapid and facile simultaneous detection of malodorous bacterial metabolites in samples of expired breath and other fluids. The invention enables estimation, by simple visual inspection and comparison against standards, of the concentration of polyamines and volatile sulfur compounds in the micromolar to millimolar range.

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42 Claims

1. (canceled)

2. A chromogenic composition useful for the detection of VSCs and polyamines, comprising DTNB and 2-IT.
- View Dependent Claims (3, 4, 5, 6, 7, 8, 18, 19, 20, 21, 23, 25, 27, 32, 38, 42)
- - 3. A composition according to claim 2 and further comprising an alkaline earth metal or a transition metal.
  - 4. A composition according to claim 3 and further comprising a buffer.
  - 5. A composition according to claim 3, wherein the metal comprises manganese, magnesium, or cobalt.
  - 6. A composition according to claim 4, wherein the pH of the composition is between about 6.3 and about 7.7.
  - 7. A composition according to claim 6, wherein the pH of the composition is about 7.
  - 8. A composition according to claim 7, wherein the shelf-life of the composition stored at room temperature is at least one year.
  - 18. A method of detecting VSCs and polyamines in a sample, said method comprising the steps of contacting the sample with the composition of claim 2 to form a mixture, and comparing the color intensity or absorbance of (a) the mixture to (b) a standard.
  - 19. A method according to claim 18, wherein the sample comprises a physiological fluid.
  - 20. A method according to claim 19, wherein the physiological fluid comprises GCF, tongue scrapings, saliva, or breath.
  - 21. A method according to claim 18, wherein the sample comprises at least one of the group consisting of (a) air, (b) water, (c) oil, and (d) a fluid in contact with a foodstuff or a food.
  - 23. A method of detecting microbial infection in or on a subject, said method comprising contacting a sample taken from the subject with the composition of claim 2 to form a mixture, and comparing the color intensity or absorbance of (a) the mixture to (b) a standard.
  - 25. A method of detecting one or more malodorous microbial metabolites in a sample of saliva or breath of a subject, said method comprising the steps of:
    - contacting the composition of claim 2 with a matrix to form an impregnated matrix;
      
      contacting the sample with the impregnated matrix to form a mixed matrix; and
      
      comparing the color intensity or absorbance of (a) the mixed matrix to (b) a standard.
  - 27. A test kit useful for the detection of a microbial metabolite, comprising the composition of claim 2.
  - 32. A composition according to claim 3, wherein the concentration of DTNB in the composition is about 0.5 millimolar, the concentration of 2-IT in the composition is about 0.5 millimolar, and the concentration of manganese chloride in the composition is about 0.1 millimolar.
  - 38. A matrix impregnated with the composition of claim 2.
  - 42. A matrix impregnated with the composition of claim 32.

9. A chromogenic composition useful for the detection of at least one of the group consisting of (a) a product of microbial metabolism, (b) a pathogenic microorganism, (c) halitosis, (d) VSC pollution, and (e) food spoilage or contamination, said composition comprising DTNB, 2-IT, a metal, and a buffer.
- View Dependent Claims (39)
- - 39. A matrix impregnated with the composition of claim 9.

10. A chromogenic composition useful for the detection of VSCs, comprising DTNB, a buffer, and at least about 0.02 millimoles of an alkaline earth metal or a transition metal per liter of the composition.
- View Dependent Claims (11, 12, 13, 40)
- - 11. A composition according to claim 10, wherein the metal comprises at least one of the group consisting of manganese, magnesium, and cobalt.
  - 12. A composition according to claim 10, wherein the pH of the composition is about 7.
  - 13. A composition according to claim 12, wherein the shelf-life of the composition is at least one year.
  - 40. A matrix impregnated with the composition of claim 10.

14-17. -17. (canceled)

22. (canceled)

24. (canceled)

26. (canceled)

28-31. -31. (canceled)

33-37. -37. (canceled)

41. (canceled)

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Current Assignee
ALT Bioscience LLC, PDX Biotech LLC
Original Assignee
ALT Bioscience LLC
Inventors
Pendergrass, James

Granted Patent

US 8,337,774 B2
Time in Patent Office

Days
Field of Search
US Class Current

435/4
CPC Class Codes

C12Q 1/04   Determining presence or kin...

G01N 31/22   using chemical indicators G...

G01N 33/52   Use of compounds or composi...

Y10T 436/173845   Amine and quaternary ammonium

Y10T 436/18   Sulfur containing

Compositions and methods for simultaneous detection of volatile sulfur compounds and polyamines

First Claim

12 Assignments

0 Petitions

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Abstract

11 Citations

42 Claims

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Compositions and methods for simultaneous detection of volatile sulfur compounds and polyamines

First Claim

12 Assignments

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0 Petitions

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Accused Products

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Abstract

11 Citations

42 Claims

Specification

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Solutions

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