In-Vitro Fertilization Systems With Spermatozoa Separated Into X-Chromosome And Y-Chromosome Bearing Populations

US 20120164733A1
Filed: 02/24/2012
Published: 06/28/2012
Est. Priority Date: 11/29/2000
Status: Active Grant

First Claim

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1-22. -22. (canceled)

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Abstract

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).

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40 Claims

1-22. -22. (canceled)

23. A method of in-vitro fertilization of oocytes, comprising:
- a) obtaining oocytes of a non-human mammal;
  
  b) obtaining sperm cells of said non-human mammal separated into an X-chromosome bearing population or a Y-chromosome bearing population;
  
  c) combining said oocytes in a fertilization medium with said sperm cells of either said X-chromosome bearing population or said Y-chromosome bearing population; and
  
  d) fertilizing at least one of said oocytes with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing population.
- View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
- - 24. The method of in-vitro fertilization of oocytes of claim 23, wherein said fertilization medium contains an amount of non-essential amino acids.
  - 25. The method of in-vitro fertilization of oocytes of claim 23, further comprising supplementing said fertilization medium with an amount of non-essential amino acids.
  - 26. The method of in-vitro fertilization of oocytes of claim 24, wherein said oocytes fertilized with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing population in said fertilization medium containing said non-essential amino acids achieve an increased rate of development through at least a two cell stage.
  - 27. The method of in-vitro fertilization of oocytes of claim 26, wherein said non-human mammal is a bovine non-human mammal, and wherein said step of achieving said increased rate of development through at least said two cell stage is selected from the group consisting of:
    - achieving at least about a 20% increase in yield of said two cell stage;
      
      achieving at least about a 45% increase in yield of said two cell stage;
      
      achieving at least about a 70% increase in yield of said two cell stage;
      
      achieving at least about a 26% yield of said two cell stage;
      
      achieving at least about a 49% yield of said two cell stage; and
      
      achieving at least about a 75% yield of said two cell stage.
  - 28. The method of in-vitro fertilization of oocytes of claim 24, wherein said oocytes fertilized with said spelin cells of said X-chromosome bearing population or said Y-chromosome bearing population in said fertilization medium containing said non-essential amino acids achieve an increased rate of blastocysts suitable for embryo transfer.
  - 29. The method of in-vitro fertilization of oocytes of claim 28, wherein said non-human mammal is a bovine non-human mammal, and wherein said step of achieving said increased rate of blastocysts suitable for embryo transfer is selected from the group consisting of:
    - achieving at least about a 9% increase in said rate of blastocysts;
      
      achieving at least about a 12% increase in said rate of blastocysts;
      
      achieving at least about a 16% increase in said rate of blastocystsachieving at least about a 52% yield of said blastocysts;
      
      achieving at least about a 61% yield of said blastocysts;
      
      achieving at least about a 64% yield of said blastocysts; and
      
      achieving at least about a 68% yield of said blastocysts.
  - 30. The method of in-vitro fertilization of oocytes of claim 28, wherein said non-human mammal comprises a bovine non-human mammal, and wherein said blastocysts suitable for embryo transfer comprise Grade 1 blastocysts.
  - 31. The method of in-vitro fertilization of oocytes of claim 23, wherein said sperm cells comprise frozen-thawed sperm cells of said non-human mammal separated into said X-chromosome bearing population or said Y-chromosome bearing population.
  - 32. The method in-vitro fertilization of bovine oocytes as described in claim 23, further comprising establishing said plurality of oocytes with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing population in incubation conditions within an incubation element.
  - 33. The method of in-vitro fertilization of oocytes of claim 32, wherein said incubation conditions comprise an atmosphere of about 5 percent carbon dioxide in air at a temperature between about 37 degrees Centigrade and about 41 degrees Centigrade for a duration of about 18 hours to about 20 hours.
  - 34. The method of in-vitro fertilization of oocytes of claim 33, further comprising transferring said oocytes and said sperm cells into a tubular member.
  - 35. The method of in-vitro fertilization of oocytes of claim 34, wherein said tubular member has heat sealable aperture elements.
  - 36. The method of in-vitro fertilization of oocytes of claim 35, wherein said tubular member has an interior volume of about 0.25 milliliters.
  - 37. The method of in-vitro fertilization of oocytes of claim 34, further comprising transferring said tubular member into an incubation element.
  - 38. The method of in-vitro fertilization of oocytes of claim 37, wherein said incubation element comprises a glass tube.
  - 39. The method of in-vitro fertilization of oocytes of claim 38, wherein said tubular member comprises an artificial insemination straw.
  - 40. The method of in-vitro fertilization of oocytes of claim 23, wherein said first and second non-human mammal is selected from the group consisting of non-human primates, bovids, ovids, equids, swine, and dolphins.

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Current Assignee
XY, LLC (Inguran LLC)
Original Assignee
XY, LLC (Inguran LLC)
Inventors
Seidel, George E., Suh, Tae Kwang, Lu, Kehuan

Granted Patent

US 8,569,053 B2
Time in Patent Office

Days
Field of Search
US Class Current

435/449
CPC Class Codes

A01N 1/02   Preservation of living parts

A01N 1/0263   Non-refrigerated containers...

A01N 1/0273   Transport containers A01N1/...

A61D 19/00   Instruments or methods for ...

C12N 5/0604   Whole embryos; Culture medi...

In-Vitro Fertilization Systems With Spermatozoa Separated Into X-Chromosome And Y-Chromosome Bearing Populations

First Claim

8 Assignments

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Accused Products

Abstract

Citations

40 Claims

Specification

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In-Vitro Fertilization Systems With Spermatozoa Separated Into X-Chromosome And Y-Chromosome Bearing Populations

First Claim

8 Assignments

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Subscription Required

0 Petitions

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Accused Products

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Abstract

Citations

40 Claims

Specification

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Solutions

Use Cases

Quick Links