Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
DC CAFC
First Claim
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1. A flow cytometer system for isolating desired cells comprising:
- a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells which contains about 2.9% sodium citrate;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed.
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Abstract
Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.
149 Citations
32 Claims
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1. A flow cytometer system for isolating desired cells comprising:
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a. a cell source which supplies cells to be analyzed by the flow cytometer; b. a sheath fluid source which creates a sheath fluid environment for said cells which contains about 2.9% sodium citrate; c. a nozzle through which said cells pass while subjected to said sheath fluid environment; d. an oscillator which acts upon said sheath fluid as it passes through said nozzle; e. a cell sensing system which responds to said cells; f. a sorter discrimination system which acts to sort cells having a desired characteristic; and g. a collector into which cells having a desired characteristic are placed. - View Dependent Claims (2, 3, 9, 10, 11)
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4. A flow cytometer system for isolating desired cells comprising:
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a. a cell source which supplies cells to be analyzed by the flow cytometer; b. a sheath fluid source which creates a sheath fluid environment for said cells which contains a hepes buffered medium; c. a nozzle through which said cells pass while subjected to said sheath fluid environment; d. an oscillator which acts upon said sheath fluid as it passes through said nozzle; e. a cell sensing system which responds to said cells; f. a sorter discrimination system which acts to sort cells having a desired characteristic; and g. a collector into which cells having a desired characteristic are placed. - View Dependent Claims (5)
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6. A flow cytometer system for isolating desired cells comprising:
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a. a cell source which supplies cells to be analyzed by the flow cytometer; b. a sheath fluid source which creates a sheath fluid environment for said cells; c. a nozzle through which said cells pass while subjected to said sheath fluid environment; d. an oscillator which acts upon said sheath fluid as it passes through said nozzle; e. a cell sensing system which responds to said cells; f. a sorter discrimination system which acts to sort cells having a desired characteristic; and g. a collector into which cells having a desired characteristic are placed and which comprises a citrate collector fluid containing about six percent egg yolk. - View Dependent Claims (7, 8)
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12. A flow cytometer system for isolating desired cells comprising:
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a. a cell source which supplies cells to be analyzed by the flow cytometer; b. a chemically coordinated sheath fluid source which creates a sheath fluid environment for said cells which is selected to be coordinated with both a pre-sort and a post-sort cell fluid environment; c. a nozzle through which said cells pass while subjected to said sheath fluid environment; d. an oscillator which acts upon said sheath fluid as it passes through said nozzle; e. a cell sensing system which responds to said cells; f. a sorter discrimination system which acts to sort cells having a desired characteristic; and g. a collector into which cells having a desired characteristic are placed. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification
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Litigation Data
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Current AssigneeXY, LLC (Inguran LLC)
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Original AssigneeXY Incorporated (Inguran LLC)
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InventorsSchenk, John, Seidel, George, Herickhoff, Lisa
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Primary Examiner(s)WALLENHORST, MAUREEN
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Application NumberUS09/001,394Time in Patent Office1,056 DaysField of Search422/68.1, 422/73, 422/82.05, 422/82.08, 422/82.09, 436/8, 436/10, 436/17, 436/18, 436/63, 436/164, 436/172, 435/283.1, 435/287.1, 435/2, 435/6, 209/571, 209/576US Class Current422/73CPC Class CodesA01K 67/027 New or modified breeds of v...A01N 1/02 Preservation of living partsA01N 1/0226 Physiologically active agen...A61D 19/02 for artificial inseminationA61D 19/04 for embryo transplantationC12N 15/873 Techniques for producing ne...C12N 5/0612 sorting of gametes, e.g. ac...C12Q 1/04 Determining presence or kin...G01N 1/30 Staining; Impregnating ; Fi...G01N 15/149 specially adapted for sorti...G01N 33/52 Use of compounds or composi...Y10T 436/101666 Particle count or volume st...Y10T 436/108331 Preservative, buffer, antic...
